Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome

DOI Web Site PubMed 被引用文献2件 オープンアクセス
  • Tanja V. Maier
    Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany
  • Marianna Lucio
    Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany
  • Lang Ho Lee
    Center for Interdisciplinary Cardiovascular Science (CICS), Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA
  • Nathan C. VerBerkmoes
    The University of Texas, El Paso, Texas, USA
  • Colin J. Brislawn
    Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
  • Jörg Bernhardt
    Institute of Microbiology, Greifswald University, Greifswald, Germany
  • Regina Lamendella
    Juniata College, Huntingdon, Pennsylvania, USA
  • Jason E. McDermott
    Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
  • Nathalie Bergeron
    Children’s Hospital Oakland Research Institute, Oakland, California, USA
  • Silke S. Heinzmann
    Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany
  • James T. Morton
    University of California, San Diego, California, USA
  • Antonio González
    University of California, San Diego, California, USA
  • Gail Ackermann
    University of California, San Diego, California, USA
  • Rob Knight
    University of California, San Diego, California, USA
  • Katharina Riedel
    Institute of Microbiology, Greifswald University, Greifswald, Germany
  • Ronald M. Krauss
    Children’s Hospital Oakland Research Institute, Oakland, California, USA
  • Philippe Schmitt-Kopplin
    Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany
  • Janet K. Jansson
    Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
  • Mary Ann Moran
    editor

書誌事項

公開日
2017-11-08
権利情報
  • https://creativecommons.org/licenses/by/4.0/
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/mbio.01343-17
公開者
American Society for Microbiology

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説明

<jats:title>ABSTRACT</jats:title><jats:p>Diet can influence the composition of the human microbiome, and yet relatively few dietary ingredients have been systematically investigated with respect to their impact on the functional potential of the microbiome. Dietary resistant starch (RS) has been shown to have health benefits, but we lack a mechanistic understanding of the metabolic processes that occur in the gut during digestion of RS. Here, we collected samples during a dietary crossover study with diets containing large or small amounts of RS. We determined the impact of RS on the gut microbiome and metabolic pathways in the gut, using a combination of “omics” approaches, including 16S rRNA gene sequencing, metaproteomics, and metabolomics. This multiomics approach captured changes in the abundance of specific bacterial species, proteins, and metabolites after a diet high in resistant starch (HRS), providing key insights into the influence of dietary interventions on the gut microbiome. The combined data showed that a high-RS diet caused an increase in the ratio of<jats:italic>Firmicutes</jats:italic>to<jats:italic>Bacteroidetes</jats:italic>, including increases in relative abundances of some specific members of the<jats:italic>Firmicutes</jats:italic>and concurrent increases in enzymatic pathways and metabolites involved in lipid metabolism in the gut.</jats:p><jats:p><jats:bold>IMPORTANCE</jats:bold>This work was undertaken to obtain a mechanistic understanding of the complex interplay between diet and the microorganisms residing in the intestine. Although it is known that gut microbes play a key role in digestion of the food that we consume, the specific contributions of different microorganisms are not well understood. In addition, the metabolic pathways and resultant products of metabolism during digestion are highly complex. To address these knowledge gaps, we used a combination of molecular approaches to determine the identities of the microorganisms in the gut during digestion of dietary starch as well as the metabolic pathways that they carry out. Together, these data provide a more complete picture of the function of the gut microbiome in digestion, including links between an RS diet and lipid metabolism and novel linkages between specific gut microbes and their metabolites and proteins produced in the gut.</jats:p>

収録刊行物

  • mBio

    mBio 8 (5), 2017-11-08

    American Society for Microbiology

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