The <scp><i>Arabidopsis</i></scp> U‐box E3 ubiquitin ligase PUB30 negatively regulates salt tolerance by facilitating BRI1 kinase inhibitor 1 (BKI1) degradation
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- Ming Zhang
- College of Agriculture Northeast Agricultural University Harbin 150030 China
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- Jinfeng Zhao
- National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing 100081 China
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- Long Li
- College of Life Sciences Shenyang Agricultural University Shenyang 110161 China
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- Yanan Gao
- College of Life Sciences Liaocheng University Liaocheng 252059 China
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- Linlin Zhao
- College of Life Sciences Liaocheng University Liaocheng 252059 China
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- Suyash Bhimgonda Patil
- National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing 100081 China
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- Jingjing Fang
- National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing 100081 China
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- Wenhui Zhang
- College of Life Sciences Liaocheng University Liaocheng 252059 China
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- Yuhong Yang
- College of Life Sciences Shenyang Agricultural University Shenyang 110161 China
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- Ming Li
- College of Agriculture Northeast Agricultural University Harbin 150030 China
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- Xueyong Li
- National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing 100081 China
説明
<jats:title>Abstract</jats:title><jats:p>The <jats:italic>Arabidopsis</jats:italic> U‐box E3 ubiquitin ligases play an important role in the ubiquitin/26S proteasome‐mediated protein degradation pathway. Recently, PUB30 has been reported to participate in the salt stress response during seed germination stage in abscisic acid (ABA)‐independent manner, but the molecular mechanism remains to be elucidated. Here, we displayed that the <jats:italic>pub30</jats:italic> mutant was more tolerant to salt stress during seed germination, whereas the mutant of its closest homologue PUB31 showed mild sensitivity to salt stress. PUB30 exhibited E3 ubiquitin ligase activity in vitro. PUB30 specifically interacted with BRI1 kinase inhibitor 1 (BKI1), a regulator playing dual roles in brassinosteroids signaling, in vitro and in vivo. We found that BKI1 protein was ubiquitinated and degraded by the 26S proteasome. The degradation of BKI1 was slowed down in the <jats:italic>pub30‐1</jats:italic> mutant compared with that in the wild type. The <jats:italic>bki1</jats:italic> mutant was sensitive to salt, whereas the transgenic plants overexpressing <jats:italic>BKI1</jats:italic> showed salt tolerant phenotype. All these results indicate that PUB30 negatively regulates salt tolerance probably through regulating the degradation of BKI1 and brassinosteroids signaling in <jats:italic>Arabidopsis</jats:italic>.</jats:p>
収録刊行物
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- Plant, Cell & Environment
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Plant, Cell & Environment 40 (11), 2831-2843, 2017-09-21
Wiley
