The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
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- Jim F Huggett
- LGC, Teddington, Middlesex, UK
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- Carole A Foy
- LGC, Teddington, Middlesex, UK
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- Vladimir Benes
- Genomics Core Facility, EMBL Heidelberg, Heidelberg, Germany
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- Kerry Emslie
- National Measurement Institute, Lindfield, Australia
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- Jeremy A Garson
- Research Department of Infection, Division of Infection and Immunity, UCL, London, UK
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- Ross Haynes
- National Institute for Standards and Technology, Gaithersburg, MD
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- Jan Hellemans
- Center for Medical Genetics Ghent (CMGG), Ghent University Hospital, Ghent, Belgium
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- Mikael Kubista
- TATAA Biocenter, Sweden, and Institute of Biotechnology of the Czech Academy of Sciences, Gothenburg, Sweden
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- Reinhold D Mueller
- Sequenom Center for Molecular Medicine, San Diego, CA
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- Tania Nolan
- Sigma Custom Products, Haverhill, Suffolk, UK
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- Michael W Pfaffl
- Physiology Weihenstephan, Center of Life and Food Sciences Weihenstephan, Germany
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- Gregory L Shipley
- Shipley Consulting, LLC, Houston, TX
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- Jo Vandesompele
- Center for Medical Genetics Ghent (CMGG), Ghent University Hospital, Ghent, Belgium
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- Carl T Wittwer
- Department of Pathology, University of Utah, Salt Lake City, UT
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- Stephen A Bustin
- Postgraduate Medical Institute, Faculty of Health, Social Care & Education, Anglia Ruskin University, Essex, UK
説明
<jats:title>Abstract</jats:title> <jats:p>There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details is required. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report addresses known requirements for dPCR that have already been identified during this early stage of its development and commercial implementation. Adoption of these guidelines by the scientific community will help to standardize experimental protocols, maximize efficient utilization of resources, and enhance the impact of this promising new technology.</jats:p>
収録刊行物
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- Clinical Chemistry
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Clinical Chemistry 59 (6), 892-902, 2013-06-01
Oxford University Press (OUP)
