Epstein–Barr virus and gastric carcinoma in Western patients: comparison of pathological parameters and p53 expression in EBV‐positive and negative tumours

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公開日
2000-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1046/j.1365-2559.2000.00843.x
公開者
Wiley

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<jats:sec><jats:title>Aims</jats:title><jats:p>The presence of Epstein–Barr virus (EBV) was studied in 56 gastric carcinomas from Western patients by in‐situ hybridization for EBV‐encoded RNAs (EBER). EBV‐positive and negative carcinomas were compared for various pathological parameters including p53 overexpression.</jats:p></jats:sec><jats:sec><jats:title>Methods and results</jats:title><jats:p>EBERs transcripts were detected in seven cases overall: four cases of 52 conventional carcinomas (7.7%) and three cases of four gastric carcinomas with lymphoid stroma (75%). EBER positivity was diffuse in five cases and restricted to a localized area of the tumour in two cases of conventional carcinoma. A monoclonal EBV genomic pattern was demonstrated in the case tested by Southern blot analysis. By immunohistochemical analysis, neither EBV latent or lytic cycle proteins nor C3d/EBV receptor were expressed by neoplastic cells. EBER positivity was significantly correlated with prominent lymphoid reaction (<jats:italic>P</jats:italic> = 0.0002) which was associated with numerous PS100‐positive dendritic cells and with HLA‐DR expression by tumour cells (<jats:italic>P</jats:italic> = 0.03). p53 immunoreactivity in more than 30% of tumour cells was detected in 25 out 49 EBV‐negative cases and was absent in EBV‐positive cases except in one case with focal EBER‐positivity.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Focal staining for EBER is an unusual finding in the setting of gastric carcinoma and these results suggest that there might be two types of EBV‐associated gastric carcinoma in which the viral infection will play a different role. The presence of a stromal lymphoid reaction which is strongly correlated with EBV positivity, is associated with antigen‐presenting ability by HLA‐DR‐positive tumour cells or abundant dendritic cells. The function of p53 appears preserved in all EBV‐associated carcinomas except in one case with focal EBER expression whereas the immunohistochemical pattern of p53 is suggestive of a mutational phenomenon in 51% of EBV‐negative cases.</jats:p></jats:sec>

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