Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field
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- Hsieh-Fu Tsai
- Institute of Biophotonics, National Yang-Ming University 1 , Taipei 11221, Taiwan
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- Shih-Wei Peng
- Institute of Biophotonics, National Yang-Ming University 1 , Taipei 11221, Taiwan
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- Chun-Ying Wu
- Research Center for Applied Sciences, Academia Sinica 2 , Taipei 11529, Taiwan
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- Hui-Fang Chang
- Research Center for Applied Sciences, Academia Sinica 2 , Taipei 11529, Taiwan
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- Ji-Yen Cheng
- Institute of Biophotonics, National Yang-Ming University 1 , Taipei 11221, Taiwan
説明
<jats:p>We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0).</jats:p>
収録刊行物
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- Biomicrofluidics
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Biomicrofluidics 6 (3), 034116-, 2012-09-01
AIP Publishing