Long-Distance Transport of Thiamine (Vitamin B1) Is Concomitant with That of Polyamines
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- Jacopo Martinis
- Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland (J.M., E.G.-P., N.S., M.C., A.G., T.B.F.); and
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- Elisabet Gas-Pascual
- Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland (J.M., E.G.-P., N.S., M.C., A.G., T.B.F.); and
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- Nicolas Szydlowski
- Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland (J.M., E.G.-P., N.S., M.C., A.G., T.B.F.); and
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- Michèle Crèvecoeur
- Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland (J.M., E.G.-P., N.S., M.C., A.G., T.B.F.); and
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- Alexandra Gisler
- Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland (J.M., E.G.-P., N.S., M.C., A.G., T.B.F.); and
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- Lukas Bürkle
- Institute of Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland (L.B.)
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- Teresa B. Fitzpatrick
- Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland (J.M., E.G.-P., N.S., M.C., A.G., T.B.F.); and
書誌事項
- 公開日
- 2016-03-22
- 権利情報
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- https://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.1104/pp.16.00009
- 公開者
- Oxford University Press (OUP)
説明
<jats:title>Abstract</jats:title><jats:p>Thiamine (vitamin B1) is ubiquitous and essential for cell energy supply in all organisms as a vital metabolic cofactor, known for over a century. In plants, it is established that biosynthesis de novo is taking place predominantly in green tissues and is furthermore limited to plastids. Therefore, transport mechanisms are required to mediate the movement of this polar metabolite from source to sink tissue to activate key enzymes in cellular energy generating pathways but are currently unknown. Similar to thiamine, polyamines are an essential set of charged molecules required for diverse aspects of growth and development, the homeostasis of which necessitates long-distance transport processes that have remained elusive. Here, a yeast-based screen allowed us to identify Arabidopsis (Arabidopsis thaliana) PUT3 as a thiamine transporter. A combination of biochemical, physiological, and genetic approaches permitted us to show that PUT3 mediates phloem transport of both thiamine and polyamines. Loss of function of PUT3 demonstrated that the tissue distribution of these metabolites is altered with growth and developmental consequences. The pivotal role of PUT3 mediated thiamine and polyamine homeostasis in plants, and its importance for plant fitness is revealed through these findings.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 171 (1), 542-553, 2016-03-22
Oxford University Press (OUP)