Diversity of Nitrous Oxide Reductase (
            <i>nosZ</i>
            ) Genes in Continental Shelf Sediments

David J. Scala, Lee J. Kerkhof

doi:10.1128/aem.65.4.1681-1687.1999

<jats:title>ABSTRACT</jats:title> <jats:p> Diversity of the nitrous oxide reductase ( <jats:italic>nosZ</jats:italic> ) gene was examined in sediments obtained from the Atlantic Ocean and Pacific Ocean continental shelves. Approximately 1,100 bp of the <jats:italic>nosZ</jats:italic> gene were amplified via PCR, using <jats:italic>nosZ</jats:italic> gene-specific primers. Thirty-seven unique copies of the <jats:italic>nosZ</jats:italic> gene from these marine environments were characterized, increasing the <jats:italic>nosZ</jats:italic> sequence database fourfold. The average DNA similarity for comparisons between all 49 variants of the <jats:italic>nosZ</jats:italic> gene was 64% ± 10%. Alignment of the derived amino acid sequences confirmed the conservation of important structural motifs. A highly conserved region is proposed as the copper binding, catalytic site (Cu <jats:sub>Z</jats:sub> ) of the mature protein. Phylogenetic analysis demonstrated three major clusters of <jats:italic>nosZ</jats:italic> genes, with little overlap between environmental and culture-based groups. Finally, the two non-culture-based gene clusters generally corresponded to sampling location, implying that denitrifier communities may be restricted geographically. </jats:p>

Diversity of Nitrous Oxide Reductase ( <i>nosZ</i> ) Genes in Continental Shelf Sediments

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