<jats:title>Abstract</jats:title><jats:sec><jats:title>Aims</jats:title><jats:p>The aim of this single‐site, randomized, controlled, double‐blind, 3‐arm parallel study was to determine the effectiveness of a prophylaxis paste containing 15% calcium sodium phosphosilicate (<jats:styled-content style="fixed-case">CSPS</jats:styled-content>; NovaMin<jats:sup>®</jats:sup>) with and without fluoride in reducing dentine hypersensitivity immediately after a single application and 28 days following dental scaling and root planing.</jats:p></jats:sec><jats:sec><jats:title>Materials & Methods</jats:title><jats:p>Overall, 151 subjects were enrolled in this study. All subjects received a scaling and root planing procedure followed by a final prophylaxis step using one of three different prophylaxis pastes: Test‐A (15% NovaMin<jats:sup>®</jats:sup> and NaF), Test‐B (15% NovaMin<jats:sup>®</jats:sup>) and a control. Dentine hypersensitivity was assessed by tactile stimulus (Yeaple Probe<jats:sup>®</jats:sup>) and by air blast (Schiff scale) at baseline, immediately after and 28 days after a prophylaxis procedure. One hundred and forty‐nine subjects completed the study.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Subjects having received the test prophylaxis pastes showed statistically lower (<jats:sc>anova</jats:sc>,<jats:italic> p</jats:italic> < 0.05) dentine hypersensitivity compared with the control group immediately after the prophylaxis procedure (Yeaple Probe<jats:sup>®</jats:sup>: Test‐A = 20.9 ± 12.6, Test‐B = 22.7 ± 12.9, Control=11.2 ± 3.1; Schiff score: Test‐A = 1.1 ± 0.6, Test‐B = 1.1 ± 0.6, Control = 2.0 ± 0.7) and after 28 days (Yeaple probe: Test‐A = 21.5 ± 11.9, Test‐B = 20.6 ± 11.3, Control = 11.8 ± 6.0; Schiff score: Test‐A = 1.0 ± 0.6, Test‐B = 1.0 ± 0.6, Control = 2.0 ± 0.7).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In conclusion, the single application of both fluoridated and non‐fluoridated prophylaxis pastes containing 15% <jats:styled-content style="fixed-case">CSPS</jats:styled-content> (NovaMin<jats:sup>®</jats:sup>) provided a significant reduction of dentine hypersensitivity up to at least 28 days.</jats:p></jats:sec>