Transformation of Amorphous Polyphosphate Nanoparticles into Coacervate Complexes: An Approach for the Encapsulation of Mesenchymal Stem Cells
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- Werner E. G. Müller
- ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6 D‐55128 Mainz Germany
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- Shunfeng Wang
- ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6 D‐55128 Mainz Germany
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- Emad Tolba
- ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6 D‐55128 Mainz Germany
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- Meik Neufurth
- ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6 D‐55128 Mainz Germany
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- Maximilian Ackermann
- Institute of Functional and Clinical Anatomy University Medical Center of the Johannes Gutenberg University Johann Joachim Becher Weg 13 D‐55099 Mainz Germany
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- Rafael Muñoz‐Espí
- Institute of Materials Science (ICMUV) Universitat de València C/Catedràtic José Beltrán 2 Paterna 46980 València Spain
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- Ingo Lieberwirth
- Max Planck Institute for Polymer Research Electron Microscopy Division Ackermannweg 10 D‐55021 Mainz Germany
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- Gunnar Glasser
- Max Planck Institute for Polymer Research Electron Microscopy Division Ackermannweg 10 D‐55021 Mainz Germany
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- Heinz C. Schröder
- ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6 D‐55128 Mainz Germany
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- Xiaohong Wang
- ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6 D‐55128 Mainz Germany
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説明
<jats:title>Abstract</jats:title><jats:p>Inorganic polyphosphate [polyP] has proven to be a promising physiological biopolymer for potential use in regenerative medicine because of its morphogenetic activity and function as an extracellular energy‐donating system. Amorphous Ca<jats:sup>2+</jats:sup>–polyP nanoparticles [Ca–polyP‐NPs] are characterized by a high zeta potential with −34 mV (at pH 7.4). This should contribute to the stability of suspensions of the spherical nanoparticles (radius 94 nm), but make them less biocompatible. The zeta potential decreases to near zero after exposure of the Ca–polyP‐NPs to protein/peptide‐containing serum or medium plus serum. Electron microscopy analysis reveals that the particles rapidly change into a coacervate phase. Those mats are amorphous, but less stable than the likewise amorphous Ca–polyP‐NPs and are morphogenetically active. Mesenchymal stem cells grown onto the polyP coacervate show enhanced growth/proliferation and become embedded in the coacervate. These results suggest that the Ca–polyP coacervate, formed from Ca–polyP‐NPs in the presence of protein, can act as an adaptable framework that mimics a niche and provides metabolic energy in bone/cartilage engineering.</jats:p>
収録刊行物
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- Small
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Small 14 (27), 2018-05-30
Wiley