Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion
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- Sandra Richter
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Marika Kientz
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Sabine Brumm
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Mads Eggert Nielsen
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Misoon Park
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Richard Gavidia
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Cornelia Krause
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Ute Voss
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Hauke Beckmann
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Ulrike Mayer
- Microscopy, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- York-Dieter Stierhof
- Microscopy, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
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- Gerd Jürgens
- Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany
説明
<jats:p>Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1–BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans-Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell–division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint.</jats:p>
収録刊行物
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- eLife
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eLife 3 e02131-, 2014-04-08
eLife Sciences Publications, Ltd
