N‐hydroxysuccinimidyl p‐methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food

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  • Fabio Mazzotti
    Dipartimento di Chimica e Tecnologie Chimiche Università della Calabria Via P. Bucci Cubo 12/C I‐87036 Arcavacata di Rende Cosenza Italy
  • Leonardo Di Donna
    Dipartimento di Chimica e Tecnologie Chimiche Università della Calabria Via P. Bucci Cubo 12/C I‐87036 Arcavacata di Rende Cosenza Italy
  • Anna Napoli
    Dipartimento di Chimica e Tecnologie Chimiche Università della Calabria Via P. Bucci Cubo 12/C I‐87036 Arcavacata di Rende Cosenza Italy
  • Donatella Aiello
    Dipartimento di Chimica e Tecnologie Chimiche Università della Calabria Via P. Bucci Cubo 12/C I‐87036 Arcavacata di Rende Cosenza Italy
  • Carlo Siciliano
    Dipartimento di Farmacia e Scienze della Salute e della Nutrizione Università della Calabria I‐87036 Arcavacata di Rende Cosenza Italy
  • Constantinos M. Athanassopoulos
    University Campus, Department of Chemistry University of Patras GR‐26500 Patras Greece
  • Giovanni Sindona
    Dipartimento di Chimica e Tecnologie Chimiche Università della Calabria Via P. Bucci Cubo 12/C I‐87036 Arcavacata di Rende Cosenza Italy

Description

<jats:p>We present a simple methodology for the simultaneous identification and determination of biogenic amines in food matrices, based on the use of a stable isotope‐coded derivatization and liquid chromatography tandem mass spectrometry. The tagging reagent is N‐hydroxysuccinimidyl ester of d<jats:sub>0</jats:sub>/d<jats:sub>4</jats:sub>‐4‐methoxybenzoic acid (d<jats:sub>0</jats:sub>/d<jats:sub>4</jats:sub>‐4‐MBA‐OSu) which mainly functionalizes primary amines. The identification and structural characterization of tagged biogenic amines were exploited by matrix‐assisted laser desorption/ionization–mass spectrometry (MS) and MS/MS. Multiple‐reaction monitoring has been applied in the assay of biogenic amines in different foodstuffs, providing a method whose reliability is confirmed by the values of accuracy (12%) and by the calculated analytical parameters. Copyright © 2014 John Wiley & Sons, Ltd.</jats:p>

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