High‐resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies
書誌事項
- 公開日
- 2009-12-16
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1755-0998.2009.02726.x
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>High‐resolution melting analysis (HRMA) is a highly sensitive closed‐tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase‐A (<jats:italic>ldh</jats:italic>‐A), myosin light chain‐2 (<jats:italic>mlc‐2</jats:italic>), acidic ribosomal phosphoprotein P0 (<jats:italic>ARP</jats:italic>) and calmodulin (<jats:italic>CaM</jats:italic>) in populations of swordfish, <jats:italic>Xiphias gladius</jats:italic>. Results indicate that HRMA is a powerful genotyping tool to study wild populations.</jats:p>
収録刊行物
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- Molecular Ecology Resources
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Molecular Ecology Resources 10 (1), 193-196, 2009-12-16
Wiley