Intact rather than total circulating insulin-like growth factor binding protein-1a is a negative indicator of growth in masu salmon
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- Nobuto Kaneko
- Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan
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- Tom Ole Nilsen
- Norwegian Research Centre (NORCE) Environment, NORCE Norwegian Research Centre AS, Bergen, Norway
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- Hanae Tanaka
- Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan
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- Akihiko Hara
- Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan
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- Munetaka Shimizu
- Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan
説明
<jats:p> Insulin-like growth factor binding protein (IGFBP)-1a is one of three major circulating forms in salmon and induced under catabolic conditions. However, there is currently no immunoassay available for this form because of a lack of standard and specific antibodies. We developed a time-resolved fluoroimmunoassay (TR-FIA) for salmon IGFBP-1a using recombinant protein for labeling, an assay standard, and production of antiserum. The TR-FIA had a low cross-reactivity (3.6%) with IGFBP-1b, another major form in the circulation. Fasting for 4 wk had no effect on serum immunoreactive (total) IGFBP-1a levels in yearling masu salmon, whereas 6-wk fasting significantly increased it. There was a significant, but weak, negative relationship between serum total IGFBP-1a level and individual growth rate ( r<jats:sup>2</jats:sup> = 0.12, P = 0.01). We next developed a ligand immuno-functional assay (LIFA) using europium-labeled IGF-I to quantify intact IGFBP-1a. In contrast to total IGFBP-1a, serum intact IGFBP-1a levels increased after 4 wk of fasting, and refeeding for 2 wk restored it to levels similar to those of the fed control. Serum intact IGFBP-1a levels showed a significant negative correlation with individual growth rate ( r<jats:sup>2</jats:sup> = 0.52, P < 0.001), which was as good as that of IGFBP-1b. Our findings using newly developed TR-FIA and LIFA suggest that regulation of intact IGFBP-1a levels has an important effect on growth in salmon and that intact IGFBP-1a is a negative index of salmon growth. </jats:p>
収録刊行物
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- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
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American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 318 (2), R329-R337, 2020-02-01
American Physiological Society
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詳細情報 詳細情報について
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- CRID
- 1361694371205933568
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- ISSN
- 15221490
- 03636119
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- データソース種別
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- Crossref
- KAKEN