Evolution and function of the neisserial <i>dam</i>‐replacing gene
書誌事項
- 公開日
- 2001-04-25
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1016/s0014-5793(01)02388-2
- 公開者
- Wiley
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説明
<jats:p>Phase variation through slippage‐like mechanisms involving homopolymeric tracts depends in part on the absence of Dam‐methylase in several pathogenic isolates of <jats:italic>Neisseria meningitidis</jats:italic>. In Dam‐defective strains <jats:italic>drg</jats:italic> (<jats:italic>dam</jats:italic>‐replacing gene), flanked by pseudo‐transposable small repeated elements (SREs), replaced <jats:italic>dam</jats:italic>. We demonstrate that <jats:italic>drg</jats:italic> encodes a restriction endonuclease (<jats:italic>Nme</jats:italic>BII) that cleaves 5′‐GmeATC‐3′. <jats:italic>drg</jats:italic> is also present in 50% of <jats:italic>Neisseria lactamica</jats:italic> strains, but in most of them it is inactive because of the absence of an SRE‐providing promoter. This is associated with the presence of GATmeC, suggesting an alternative restriction‐modification system (RM) specific for 5′‐GATC‐3′, similar to <jats:italic>Sau</jats:italic>3AI‐RM of <jats:italic>Staphylococcus aureus</jats:italic> 3A, <jats:italic>Lactococcus lactis</jats:italic> KR2 and <jats:italic>Listeria monocytogenes</jats:italic>.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 495 (3), 178-183, 2001-04-25
Wiley
