Studies on the immunogenicity of a recombinant ookinete surface antigen Pbs21 from <i>Plasmodium berghei</i> expressed in <i>Escherichia coli</i>
書誌事項
- 公開日
- 1994-01
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1365-3024.1994.tb00301.x
- 公開者
- Wiley
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説明
<jats:title>SUMMARY</jats:title><jats:p> <jats:italic>Plasmodium berghei</jats:italic> ookinete surface antigen (Pbs21), was produced as a fusion product with maltose binding protein (MBP) in <jats:italic>Escherichia coli</jats:italic> and used to induce transmission‐blocking immunity in mice. Specificity of induced antibody was confirmed by Western blotting with native ookinete Pbs21, and by the indirect immunofluorescent antibody test on ookinete bloodfilms. Immunized mice were infected with <jats:italic>P. berghei</jats:italic> and transmission to <jats:italic>Anopheles stephensi</jats:italic> mosquitoes determined by both the intensity and prevalence of oocyst infections. Compared with a control group immunized with MBP alone the maximum blockade of oocyst intensity was 66% in the mice immunized with recombinant MBP‐Pbs21. Over nine experiments blockade averaged only 33%. By comparison with native Pbs21 protein, which usually induces 90% blockade, our data suggests the recombinant protein produced in this bacterial system is a less effective immunogen despite expressing epitopes recognized by known transmission‐blocking monoclonal antibodies.</jats:p>
収録刊行物
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- Parasite Immunology
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Parasite Immunology 16 (1), 27-34, 1994-01
Wiley
