Dodeca-CLE Peptides as Suppressors of Plant Stem Cell Differentiation

  • Yasuko Ito
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Ikuko Nakanomyo
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Hiroyasu Motose
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Kuninori Iwamoto
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Shinichiro Sawa
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Naoshi Dohmae
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Hiroo Fukuda
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

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Description

<jats:p> In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the <jats:italic>CLE</jats:italic> family, which includes <jats:italic>CLAVATA3</jats:italic> in <jats:italic>Arabidopsis</jats:italic> . The dodecapeptide suppresses xylem cell development at a concentration of 10 <jats:sup>–11</jats:sup> M and promotes cell division. An application, corresponding to all 26 <jats:italic>Arabidopsis</jats:italic> CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate. </jats:p>

Journal

  • Science

    Science 313 (5788), 842-845, 2006-08-11

    American Association for the Advancement of Science (AAAS)

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