Transgenic rice (<i>Oryza sativa</i>) endosperm expressing daffodil (<i>Narcissus pseudonarcissus</i>) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis

DOI Web Site Web Site 被引用文献10件

この論文をさがす

説明

<jats:p>Rice (<jats:italic>Oryza sativa</jats:italic> L.), the major food staple for more than two billion people, contains neither β‐carotene (provitamin A) nor C<jats:sub>40</jats:sub> carotenoid precursors thereof in its endosperm. To improve the nutritional value of rice, genetic engineering was chosen as a means to introduce the ability to make β‐carotene into rice endosperm tissue. Investigation of the biochemical properties of immature rice endosperm using [<jats:sup>14</jats:sup>C]‐labelled substrates revealed the presence of geranyl geranyl diphosphate, the C<jats:sub>20</jats:sub> general isoprenoid precursor necessary for C<jats:sub>40</jats:sub> carotenoid biosynthesis. Phytoene synthase, which condenses two molecules of geranyl geranyl diphosphate, is the first of four specific enzymes necessary for β‐carotene biosynthesis in plants. Therefore, the Japonica rice model variety Taipei 309 was transformed by microprojectile bombardment with a cDNA coding for phytoene synthase from daffodil (<jats:italic>Narcissus pseudonarcissus</jats:italic>) under the control of either a constitutive or an endosperm‐specific promoter. In transgenic rice plants, the daffodil enzyme is active, as measured by the <jats:italic>in vivo</jats:italic> accumulation of phytoene in rice endosperm. Thus, it is demonstrated for the first time that it is in principle possible to engineer a critical step in provitamin A biosynthesis in a non‐photosynthetic, carotenoid‐lacking plant tissue. These results have important implications for longterm prospects of overcoming worldwide vitamin A deficiency.</jats:p>

収録刊行物

被引用文献 (10)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ