Mouse and human HSPC immobilization in liquid culture by CD43- or CD44-antibody coating
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- Dirk Loeffler
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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- Weijia Wang
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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- Alois Hopf
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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- Oliver Hilsenbeck
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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- Paul E. Bourgine
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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- Fabian Rudolf
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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- Ivan Martin
- Department of Biomedicine, University Hospital Basel/University of Basel, Basel, Switzerland
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- Timm Schroeder
- Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland; and
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説明
<jats:title>Key Points</jats:title> <jats:p>Anti-CD43– and anti-CD44–antibody coating immobilizes live mouse and human HSPCs. This enables 2D colony formation, medium exchange without cell-identification loss, and increased throughput of time-lapse imaging.</jats:p>
収録刊行物
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- Blood
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Blood 131 (13), 1425-1429, 2018-03-29
American Society of Hematology