Cloning and Identification of a MicroRNA Cluster within the Latency-Associated Region of Kaposi's Sarcoma-Associated Herpesvirus

DOI Web Site 3 Citations
  • Mark A. Samols
    Department of Molecular Genetics and Microbiology and UF Shands Cancer Center, University of Florida, Gainesville, Florida 32610
  • Jianhong Hu
    Department of Molecular Genetics and Microbiology and UF Shands Cancer Center, University of Florida, Gainesville, Florida 32610
  • Rebecca L. Skalsky
    Department of Molecular Genetics and Microbiology and UF Shands Cancer Center, University of Florida, Gainesville, Florida 32610
  • Rolf Renne
    Department of Molecular Genetics and Microbiology and UF Shands Cancer Center, University of Florida, Gainesville, Florida 32610

Description

<jats:title>ABSTRACT</jats:title> <jats:p>MicroRNAs (miRNAs) are small, noncoding regulatory RNA molecules that bind to 3′ untranslated regions (UTRs) of mRNAs to either prevent their translation or induce their degradation. Previously identified in a variety of organisms ranging from plants to mammals, miRNAs are also now known to be produced by viruses. The human gammaherpesvirus Epstein-Barr virus has been shown to encode miRNAs, which potentially regulate both viral and cellular genes. To determine whether Kaposi's sarcoma-associated herpesvirus (KSHV) encodes miRNAs, we cloned small RNAs from KSHV-positive primary effusion lymphoma-derived cells and endothelial cells. Sequence analysis revealed 11 isolated RNAs of 19 to 23 bases in length that perfectly align with KSHV. Surprisingly, all candidate miRNAs mapped to a single genomic locale within the latency-associated region of KSHV. These data suggest that viral and host cellular gene expression may be regulated by miRNAs during both latent and lytic KSHV replication.</jats:p>

Journal

  • Journal of Virology

    Journal of Virology 79 (14), 9301-9305, 2005-07

    American Society for Microbiology

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