Recent Advances in the Polymerase Chain Reaction

  • Henry A. Erlich
    Department of Human Genetics, Core Technology, and Department of Infectious Diseases, Cetus Corporation, Emeryville, CA 94608.
  • David Gelfand
    Department of Human Genetics, Core Technology, and Department of Infectious Diseases, Cetus Corporation, Emeryville, CA 94608.
  • John J. Sninsky
    Department of Human Genetics, Core Technology, and Department of Infectious Diseases, Cetus Corporation, Emeryville, CA 94608.

書誌事項

公開日
1991-06-21
DOI
  • 10.1126/science.2047872
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p>The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.</jats:p>

収録刊行物

  • Science

    Science 252 (5013), 1643-1651, 1991-06-21

    American Association for the Advancement of Science (AAAS)

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