説明
<jats:p>Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The method can introduce small mutations into promoter sites and is even better suited for introducing single or double mutations into proteins. It is elegant in its simplicity and can be applied quite easily in any laboratory using standard protein expression vectors and commercially available reagents.</jats:p>
収録刊行物
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- Cold Spring Harbor Protocols
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Cold Spring Harbor Protocols 2013 (8), pdb.prot076505-, 2013-08
Cold Spring Harbor Laboratory
