Plasma MicroRNA 499 as a Biomarker of Acute Myocardial Infarction

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書誌事項

公開日
2010-07-01
権利情報
  • https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
DOI
  • 10.1373/clinchem.2010.144121
公開者
Oxford University Press (OUP)

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<jats:title>Abstract</jats:title> <jats:p>Background: MicroRNAs (miRNAs) are endogenous small RNAs 21–25 nucleotides in length. Recently, we reported that miRNA 208 (miR-208) is produced exclusively in the rat myocardium and that plasma miR-208 is a biomarker of myocardial injury in rats. In the present study, we assessed the hypothesis that plasma concentrations of myocardial-specific miRNAs can be used to diagnose myocardial injury in humans.</jats:p> <jats:p>Methods: We used array analysis of miRNA production in various human tissues to identify heart-specific miRNAs. We assessed the plasma concentrations of miR-499 in 14 individuals with acute coronary syndromes, 15 individuals with congestive heart failure, and 10 individuals without cardiovascular diseases. Plasma miR-499 concentrations were measured with a real-time reverse-transcription PCR method that used an artificial small RNA as an internal calibrator.</jats:p> <jats:p>Results: The miRNA array analysis of various human tissues indicated that miR-499 was produced almost exclusively in the heart. Plasma miR-499 concentrations were measurably increased in all individuals with acute myocardial infarction but were below the limit of detection for all individuals in the other patient groups.</jats:p> <jats:p>Conclusions: The plasma concentration of miR-499 may be a useful biomarker of myocardial infarction in humans.</jats:p>

収録刊行物

  • Clinical Chemistry

    Clinical Chemistry 56 (7), 1183-1185, 2010-07-01

    Oxford University Press (OUP)

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