Tri‐n‐butyltin Increases Intracellular Ca<sup>2+</sup> in Mouse Thymocytes: A Flow‐Cytometric Study Using Fluorescent Dyes for Membrane Potential and Intracellular Ca<sup>2+</sup>
書誌事項
- 公開日
- 1992-09
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1600-0773.1992.tb00543.x
- 公開者
- Wiley
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説明
<jats:p><jats:bold>Abstract: </jats:bold> Effects of tri‐n‐butyltin (TBT) on mouse thymocytes were examined using a flow‐cytometer and fluorescent dyes for membrane potential and intracellular Ca<jats:sup>2+</jats:sup> ([Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>). TBT at concentrations from 1 × 10<jats:sup>‐7</jats:sup> M to 3 × 10<jats:sup>‐7</jats:sup> M caused hyperpolarization in thymocytes during 30 min. after drug application in a time‐ and dose‐dependent manner. Further increase in TBT concentration (to 1 × 10<jats:sup>‐6</jats:sup> M) made hyperpolarization of thymocytes more profound within 5 min. after application, thereafter gradually depolarized them during the next 25 min. TBT at 3 × 10<jats:sup>‐8</jats:sup> M or more (up to 1 × 10<jats:sup>‐6</jats:sup> M) increased the [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> of thymocytes. After reaching maximum [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> at the various TBT concentrations used within 5 min. after drug application, the [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> slightly decreased in a time‐dependent manner. Effects of TBT on membrane potential and the [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> were greatly reduced under nominal external Ca<jats:sup>2+</jats:sup> ‐free condition. Results suggest that TBT can promote Ca<jats:sup>2+</jats:sup> ‐influx to thymocytes, resulting in hyperpolarization by activation of Ca<jats:sup>2+</jats:sup> ‐dependent K<jats:sup>+</jats:sup> current. The increase in [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> by TBT may be related to its cytotoxic action on thymocytes.</jats:p>
収録刊行物
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- Pharmacology & Toxicology
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Pharmacology & Toxicology 71 (3), 190-195, 1992-09
Wiley