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- Kerstin Juelke
- Innate Immunity, Deutsches Rheuma Forschungszentrum (DRFZ), Berlin, Germany;
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- Monica Killig
- Innate Immunity, Deutsches Rheuma Forschungszentrum (DRFZ), Berlin, Germany;
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- Merlin Luetke-Eversloh
- Innate Immunity, Deutsches Rheuma Forschungszentrum (DRFZ), Berlin, Germany;
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- Eliana Parente
- Dipartimento di Fisiopatologia Clinica, Excellence Center for Research, Transfer and High Education, Denothe University of Florence, Florence, Italy;
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- Joachim Gruen
- Bioinformatics, DRFZ, Berlin, Germany;
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- Barbara Morandi
- Laboratory of Immunology and Biotherapy, Department of Human Pathology, University of Messina, Messina, Italy; and
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- Guido Ferlazzo
- Laboratory of Immunology and Biotherapy, Department of Human Pathology, University of Messina, Messina, Italy; and
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- Andreas Thiel
- Berlin-Brandenburg Centre for Regenerative Therapies, Charité-University Medicine, Berlin, Germany;
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- Isabela Schmitt-Knosalla
- Institute of Medical Immunology, Charité-University Medicine Berlin, Berlin, Germany
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- Chiara Romagnani
- Innate Immunity, Deutsches Rheuma Forschungszentrum (DRFZ), Berlin, Germany;
書誌事項
- 公開日
- 2010-08-26
- DOI
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- 10.1182/blood-2009-11-253286
- 公開者
- American Society of Hematology
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>Human natural killer (NK) cells comprise 2 main subsets, CD56bright and CD56dim cells, that differ in function, phenotype, and tissue localization. To further dissect the heterogeneity of CD56dim cells, we have performed transcriptome analysis and functional ex vivo characterization of human NK-cell subsets according to the expression of markers related to differentiation, migration or competence. Here, we show for the first time that the ability to respond to cytokines or to activating receptors is mutually exclusive in almost all NK cells with the exception of CD56dim CD62L+ cells. Indeed, only these cells combine the ability to produce interferon-γ after cytokines and proliferate in vivo during viral infection with the capacity to kill and produce cytokines upon engagement of activating receptors. Therefore, CD56dim CD62L+ cells represent a unique subset of polyfunctional NK cells. Ex vivo analysis of their function, phenotype, telomere length, frequencies during ageing as well as transfer experiments of NK-cell subsets into immunodeficient mice suggest that CD56dim CD62L+ cells represent an intermediate stage of NK-cell maturation, which after restimulation can accomplish multiple tasks and further develop into terminally differentiated effectors.</jats:p>
収録刊行物
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- Blood
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Blood 116 (8), 1299-1307, 2010-08-26
American Society of Hematology