Determination of enzyme activities of energy metabolism in the maturing rat oocyte
書誌事項
- 公開日
- 1992-11
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/mrd.1080330315
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6‐P and 6‐P gluconate dehydrogenases, were characteristic of immature oocytes. Hexokinase may be a rate‐limiting enzyme that enables oocytes to use glucose as an energy source. During maturation, the activities of hexokinase, phospho‐fructokinase, and malate dehydrogenase increased significantly, suggesting that the glycolytic pathway, as well as the tricarboxylic acid cycle, developed as the first meiotic division proceeded. In contrast, the activities of glucose 6‐P and 6‐P gluconate dehydrogenases decreased in maturing oocytes. The observation that the enzyme pattern in mature oocytes resembles more closely that in somatic cells appears to be significant, especially in light of previous studies showing this developmental trend in preimplantation embryos. © 1992 Wiley‐Liss, Inc.</jats:p>
収録刊行物
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- Molecular Reproduction and Development
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Molecular Reproduction and Development 33 (3), 333-337, 1992-11
Wiley