A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly
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- S. O. Dodonova
- Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
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- P. Diestelkoetter-Bachert
- Heidelberg University Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
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- A. von Appen
- Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
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- W. J. H. Hagen
- Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
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- R. Beck
- Heidelberg University Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
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- M. Beck
- Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
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- F. Wieland
- Heidelberg University Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
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- J. A. G. Briggs
- Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
書誌事項
- 公開日
- 2015-07-10
- 権利情報
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- http://www.sciencemag.org/about/science-licenses-journal-article-reuse
- DOI
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- 10.1126/science.aab1121
- 公開者
- American Association for the Advancement of Science (AAAS)
この論文をさがす
説明
<jats:title>A coat of many components</jats:title> <jats:p> The formation of coated trafficking vesicles is among the most fundamental of cellular processes. COP1 transport vesicles are involved in retrograde membrane trafficking in the Golgi apparatus and endoplasmic reticulum. Dodonova <jats:italic>et al.</jats:italic> applied cryo–electron tomography to determine the structure of the COPI coat in its fully assembled form on budded vesicles (see the Perspective by Noble and Stagg). They combined structural data with cross-linking mass spectrometry to generate a complete molecular model. The model suggests a mechanism of coat assembly in which coat proteins cluster via flexible interactions instead of forming a protein cage on the membrane. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6244" page="195" related-article-type="in-this-issue" vol="349" xlink:href="10.1126/science.aab1121">195</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6244" page="142" related-article-type="in-this-issue" vol="349" xlink:href="10.1126/science.aac6537">142</jats:related-article> </jats:p>
収録刊行物
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- Science
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Science 349 (6244), 195-198, 2015-07-10
American Association for the Advancement of Science (AAAS)