Reversible inhibition of mammalian glutamine synthetase by tyrosine nitration

Boris Görg, Natalia Qvartskhava, Peter Voss, Tilman Grune, Dieter Häussinger, Freimut Schliess

doi:10.1016/j.febslet.2006.11.081

Reversible inhibition of mammalian glutamine synthetase by tyrosine nitration

DOI Web Site Web Site 被引用文献2件

書誌事項

公開日: 2006-12-12

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http://onlinelibrary.wiley.com/termsAndConditions#vor

DOI

10.1016/j.febslet.2006.11.081

公開者: Wiley

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<jats:p>The effect of tyrosine nitration on mammalian GS activity and stability was studied in vitro. Peroxynitrite at a concentration of 5 μmol/l produced tyrosine nitration and inactivation of GS, whereas 50 μmol/l peroxynitrite additionally increased <jats:italic>S</jats:italic>‐nitrosylation and carbonylation and degradation of GS by the 20S proteasome. (−)Epicatechin completely prevented both, tyrosine nitration and inactivation of GS by peroxynitrite (5 μmol/l). Further, a putative “denitrase” activity restored the activity of peroxynitrite (5 μmol/l)‐treated GS. The data point to a potential regulation of GS activity by a reversible tyrosine nitration. High levels of oxidative stress may irreversibly damage and predispose the enzyme to proteasomal degradation.</jats:p>