Depth-specific optogenetic control in vivo with a scalable, high-density μLED neural probe
抄録
<jats:title>Abstract</jats:title><jats:p>Controlling neural circuits is a powerful approach to uncover a causal link between neural activity and behaviour. Optogenetics has been widely adopted by the neuroscience community as it offers cell-type-specific perturbation with millisecond precision. However, these studies require light delivery in complex patterns with cellular-scale resolution, while covering a large volume of tissue at depth <jats:italic>in vivo</jats:italic>. Here we describe a novel high-density silicon-based microscale light-emitting diode (μLED) array, consisting of up to ninety-six 25 μm-diameter μLEDs emitting at a wavelength of 450 nm with a peak irradiance of 400 mW/mm<jats:sup>2</jats:sup>. A width of 100 μm, tapering to a 1 μm point, and a 40 μm thickness help minimise tissue damage during insertion. Thermal properties permit a set of optogenetic operating regimes, with ~0.5 °C average temperature increase. We demonstrate depth-dependent activation of mouse neocortical neurons <jats:italic>in vivo</jats:italic>, offering an inexpensive novel tool for the precise manipulation of neural activity.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 6 (1), 28381-, 2016-06-23
Springer Science and Business Media LLC
