Characterization of maize ( <i>Zea mays</i> L.) Wee1 and its activity in developing endosperm
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- Yuejin Sun
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Brian P. Dilkes
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Chunsheng Zhang
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Ricardo A. Dante
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Newton P. Carneiro
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Keith S. Lowe
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Rudolf Jung
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- William J. Gordon-Kamm
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
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- Brian A. Larkins
- Department of Plant Sciences, University of Arizona, Tucson, AZ 85721; and Pioneer Hi-Bred International, Inc., Johnston, IA 50131
書誌事項
- 公開日
- 1999-03-30
- DOI
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- 10.1073/pnas.96.7.4180
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p> We report the characterization of a maize Wee1 homologue and its expression in developing endosperm. Using a 0.8-kb cDNA from an expressed sequence tag project, we isolated a 1.6-kb cDNA (ZmWee1), which encodes a protein of 403 aa with a calculated molecular size of 45.6 kDa. The deduced amino acid sequence shows 50% identity to the protein kinase domain of human Wee1. Overexpression of ZmWee1 in <jats:italic>Schizosaccharomyces pombe</jats:italic> inhibited cell division and caused the cells to enlarge significantly. Recombinant ZmWee1 obtained from <jats:italic>Escherichia coli</jats:italic> is able to inhibit the activity of p13 <jats:sup>suc1</jats:sup> -adsorbed cyclin-dependent kinase from maize. ZmWee1 is encoded by a single gene at a locus on the long arm of chromosome 4. RNA gel blots showed the ZmWee1 transcript is about 2.4 kb in length and that its abundance reaches a maximum 15 days after pollination in endosperm tissue. High levels of expression of ZmWee1 at this stage of endosperm development imply that ZmWee1 plays a role in endoreduplication. Our results show that control of cyclin-dependent kinase activity by Wee1 is conserved among eukaryotes, from fungi to animals and plants. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 96 (7), 4180-4185, 1999-03-30
Proceedings of the National Academy of Sciences