Large-Scale Selection and Breeding To Generate Industrial Yeasts with Superior Aroma Production

  • Jan Steensels
    Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, and Laboratory for Systems Biology, VIB, Bio-Incubator, Leuven, Belgium
  • Esther Meersman
    Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, and Laboratory for Systems Biology, VIB, Bio-Incubator, Leuven, Belgium
  • Tim Snoek
    Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, and Laboratory for Systems Biology, VIB, Bio-Incubator, Leuven, Belgium
  • Veerle Saels
    Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, and Laboratory for Systems Biology, VIB, Bio-Incubator, Leuven, Belgium
  • Kevin J. Verstrepen
    Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, and Laboratory for Systems Biology, VIB, Bio-Incubator, Leuven, Belgium

説明

<jats:title>ABSTRACT</jats:title> <jats:p> The concentrations and relative ratios of various aroma compounds produced by fermenting yeast cells are essential for the sensory quality of many fermented foods, including beer, bread, wine, and sake. Since the production of these aroma-active compounds varies highly among different yeast strains, careful selection of variants with optimal aromatic profiles is of crucial importance for a high-quality end product. This study evaluates the production of different aroma-active compounds in 301 different <jats:named-content content-type="genus-species">Saccharomyces cerevisiae</jats:named-content> , <jats:named-content content-type="genus-species">Saccharomyces paradoxus</jats:named-content> , and <jats:named-content content-type="genus-species">Saccharomyces pastorianus</jats:named-content> yeast strains. Our results show that the production of key aroma compounds like isoamyl acetate and ethyl acetate varies by an order of magnitude between natural yeasts, with the concentrations of some compounds showing significant positive correlation, whereas others vary independently. Targeted hybridization of some of the best aroma-producing strains yielded 46 intraspecific hybrids, of which some show a distinct heterosis (hybrid vigor) effect and produce up to 45% more isoamyl acetate than the best parental strains while retaining their overall fermentation performance. Together, our results demonstrate the potential of large-scale outbreeding to obtain superior industrial yeasts that are directly applicable for commercial use. </jats:p>

収録刊行物

被引用文献 (2)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ