Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells
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- M R Hoda
- Division of Gastroenterology, Department of Medicine, University of California, San Diego, School of Medicine, San Diego, California, USA
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- S J Keely
- Division of Gastroenterology, Department of Medicine, University of California, San Diego, School of Medicine, San Diego, California, USA
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- L S Bertelsen
- Division of Gastroenterology, Department of Medicine, University of California, San Diego, School of Medicine, San Diego, California, USA
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- W G Junger
- Division of Trauma Surgery, Department of Surgery, University of California, San Diego, School of Medicine, San Diego, California, USA
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- D Dharmasena
- Division of Gastroenterology, Department of Medicine, University of California, San Diego, School of Medicine, San Diego, California, USA
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- K E Barrett
- Division of Gastroenterology, Department of Medicine, University of California, San Diego, School of Medicine, San Diego, California, USA
説明
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Obesity is associated with increased levels of leptin. The mitogenic actions of leptin have been identified in various cell types. Because obesity may be a risk factor for colonic cancer, the proliferative and antiapoptotic effects of leptin on colonic cancer cells and the role of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3-K) signalling were investigated.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Three human colonic cancer cell lines (T84, HT29/Cl.19A and Caco-2) were treated with leptin. Cell proliferation was measured using the XTT® colorimetric assay and apoptosis by a cell death enzyme-linked immunosorbent assay. Inhibitors of MAPK and PI3-K were used to evaluate the role of these signalling pathways. Phosphorylation of the downstream components extracellular signal-regulated kinase (ERK) 1/2 and Akt was detected by western blotting.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Leptin increased cell number in all cell lines in a dose-dependent manner and reduced the number of apoptotic cells in a cell line-dependent manner. Leptin also caused ERK1/2 and Akt phosphorylation. Pretreatment with inhibitors of MAPK and PI3-K inhibited these responses, attenuated the mitogenic action of leptin and abolished its antiapoptotic effects.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Chronic increases in leptin concentration may enhance the growth of colonic cancers via MAPK and PI3-K pathways. These effects of leptin could provide a link between obesity and colonic cancer, and may represent a target for anticancer drug development.</jats:p> </jats:sec>
収録刊行物
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- British Journal of Surgery
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British Journal of Surgery 94 (3), 346-354, 2007-01-10
Oxford University Press (OUP)
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キーワード
詳細情報 詳細情報について
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- CRID
- 1361699994659616896
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- DOI
- 10.1002/bjs.5530
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- ISSN
- 13652168
- 00071323
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- データソース種別
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- Crossref