The Effect of Human and Mouse Fibroblast Feeder Cells on Cardiac Differentiation of Human Pluripotent Stem Cells
-
- Mari Pekkanen-Mattila
- Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
-
- Marisa Ojala
- Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
-
- Erja Kerkelä
- Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
-
- Kristiina Rajala
- Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
-
- Heli Skottman
- Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
-
- Katriina Aalto-Setälä
- Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland
この論文をさがす
説明
<jats:p>Mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (hFFs) are commonly used as feeder cells to maintain the pluripotent state of stem cells. The aim of the present study was to evaluate the effect of MEF and hFF feeders on the cardiac differentiation. Two human embryonic and two induced pluripotent stem cell lines were cultured on MEF and hFF before cardiac differentiation. The expression of Brachyury T was higher in cell lines cultured on MEF, than if cultured on hFF, suggesting enhanced mesoderm formation. However, significant positive influence of MEF feeders on cardiac differentiation was only seen with one cell line. Further, the ability of hFF to maintain pluripotency of stem cells originally cultured on MEF was quite poor. In conclusion, the cells behaved differently whether cultured on hFF or MEF feeders. However, the influence of the feeder cells on differentiation was less than the difference observed between the cell lines.</jats:p>
収録刊行物
-
- Stem Cells International
-
Stem Cells International 2012 1-10, 2012
Hindawi Limited