Identification of restriction fragment length polymorphisms in DNA from Mycobacterium paratuberculosis
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- D Whipple
- Leptospirosis/Mycobacteriosis Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.
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- P Kapke
- Leptospirosis/Mycobacteriosis Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.
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- C Vary
- Leptospirosis/Mycobacteriosis Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.
書誌事項
- 公開日
- 1990-11
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/jcm.28.11.2561-2564.1990
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:p>DNAs from 34 mycobactin-dependent isolates of Mycobacterium paratuberculosis and 1 isolate of M. paratuberculosis 18 were digested with four restriction endonucleases. Southern hybridization experiments were performed with a 32P-labeled oligonucleotide DNA probe derived from the sequence of IS900, an insertion sequence present in 15 to 20 copies per M. paratuberculosis chromosome. The probe hybridized with DNA from each of the mycobactin-dependent isolates, and restriction fragment length polymorphisms were detected among the isolates with each restriction endonuclease used. Restriction fragment length polymorphism analysis may permit identification of various strains of M. paratuberculosis, which has not been possible with other techniques.</jats:p>
収録刊行物
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- Journal of Clinical Microbiology
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Journal of Clinical Microbiology 28 (11), 2561-2564, 1990-11
American Society for Microbiology
