Overexpression of a BAHD Acyltransferase, <i>OsAt10</i>, Alters Rice Cell Wall Hydroxycinnamic Acid Content and Saccharification

  • Laura E. Bartley
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Matthew L. Peck
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Sung-Ryul Kim
    Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446–701, Republic of Korea (S.-R.K., G.A., P.C.R.)
  • Berit Ebert
    Joint BioEnergy Institute, Emeryville, California 94608 (L.E.B., B.E., C.M., D.M.C., C.R., M.E.V.-S., P.I.B., P.C., J.D.K., H.V.S., P.C.R.)
  • Chithra Manisseri
    Joint BioEnergy Institute, Emeryville, California 94608 (L.E.B., B.E., C.M., D.M.C., C.R., M.E.V.-S., P.I.B., P.C., J.D.K., H.V.S., P.C.R.)
  • Dawn M. Chiniquy
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)
  • Robert Sykes
    BioEnergy Sciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401 (R.S., A.Z.)
  • Lingfang Gao
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Carsten Rautengarten
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)
  • Miguel E. Vega-Sánchez
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)
  • Peter I. Benke
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Patrick E. Canlas
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)
  • Peijian Cao
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)
  • Susan Brewer
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)
  • Fan Lin
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Whitney L. Smith
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Xiaohan Zhang
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Jay D. Keasling
    Joint BioEnergy Institute, Emeryville, California 94608 (L.E.B., B.E., C.M., D.M.C., C.R., M.E.V.-S., P.I.B., P.C., J.D.K., H.V.S., P.C.R.)
  • Rolf E. Jentoff
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Steven B. Foster
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Jizhong Zhou
    Department of Microbiology and Plant Biology (L.E.B., M.L.P., L.G., F.L., J.Z.), Department of Chemistry and Biochemistry (W.L.S., S.B.F.), and Department of Chemical, Biological, and Materials Engineering (X.Z., R.E.J.), University of Oklahoma, Norman, Oklahoma 73019
  • Angela Ziebell
    BioEnergy Sciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401 (R.S., A.Z.)
  • Gynheung An
    Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446–701, Republic of Korea (S.-R.K., G.A., P.C.R.)
  • Henrik V. Scheller
    Joint BioEnergy Institute, Emeryville, California 94608 (L.E.B., B.E., C.M., D.M.C., C.R., M.E.V.-S., P.I.B., P.C., J.D.K., H.V.S., P.C.R.)
  • Pamela C. Ronald
    Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616 (L.E.B., D.M.C., M.E.V.-S., P.E.C., P.C., S.B., P.C.R.)

抄録

<jats:title>Abstract</jats:title> <jats:p>Grass cell wall properties influence food, feed, and biofuel feedstock usage efficiency. The glucuronoarabinoxylan of grass cell walls is esterified with the phenylpropanoid-derived hydroxycinnamic acids ferulic acid (FA) and para-coumaric acid (p-CA). Feruloyl esters undergo oxidative coupling with neighboring phenylpropanoids on glucuronoarabinoxylan and lignin. Examination of rice (Oryza sativa) mutants in a grass-expanded and -diverged clade of BAHD acyl-coenzyme A-utilizing transferases identified four mutants with altered cell wall FA or p-CA contents. Here, we report on the effects of overexpressing one of these genes, OsAt10 (LOC_Os06g39390), in rice. An activation-tagged line, OsAT10-D1, shows a 60% reduction in matrix polysaccharide-bound FA and an approximately 300% increase in p-CA in young leaf tissue but no discernible phenotypic alterations in vegetative development, lignin content, or lignin composition. Two additional independent OsAt10 overexpression lines show similar changes in FA and p-CA content. Cell wall fractionation and liquid chromatography-mass spectrometry experiments isolate the cell wall alterations in the mutant to ester conjugates of a five-carbon sugar with p-CA and FA. These results suggest that OsAT10 is a p-coumaroyl coenzyme A transferase involved in glucuronoarabinoxylan modification. Biomass from OsAT10-D1 exhibits a 20% to 40% increase in saccharification yield depending on the assay. Thus, OsAt10 is an attractive target for improving grass cell wall quality for fuel and animal feed.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 161 (4), 1615-1633, 2013-02-07

    Oxford University Press (OUP)

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