TMG-chitotriomycin as a probe for the prediction of substrate specificity of β-N-acetylhexosaminidases
書誌事項
- 公開日
- 2013-06
- DOI
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- 10.1016/j.carres.2013.04.024
- 公開者
- Elsevier BV
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説明
TMG-chitotriomycin (1) produced by the actinomycete Streptomyces annulatus NBRC13369 was examined as a probe for the prediction of substrate specificity of β-N-acetylhexosaminidases (HexNAcases). According to the results of inhibition assays, 14 GH20 HexNAcases from various organisms were divided into 1-sensitive and 1-insensitive enzymes. Three representatives of each group were investigated for their substrate specificity. The 1-sensitive HexNAcases hydrolyzed N-acetylchitooligosaccharides but not N-glycan-type oligosaccharides, whereas the 1-insensitive enzymes hydrolyzed N-glycan-type oligosaccharides but not N-acetylchitooligosaccharides, indicating that TMG-chitotriomycin can be used as a molecular probe to distinguish between chitin-degrading HexNAcases and glycoconjugate-processing HexNAcases.
収録刊行物
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- Carbohydrate Research
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Carbohydrate Research 375 29-34, 2013-06
Elsevier BV
