Interbase FRET in RNA: from A to Z

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  • Anders F Füchtbauer
    Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
  • Moa S Wranne
    Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
  • Mattias Bood
    Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-412 96, Sweden
  • Erik Weis
    Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-412 96, Sweden
  • Pauline Pfeiffer
    Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
  • Jesper R Nilsson
    Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
  • Anders Dahlén
    Medicinal Chemistry, Research and Early Development Cardiovascular, Renal and Metabolism, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
  • Morten Grøtli
    Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-412 96, Sweden
  • L Marcus Wilhelmsson
    Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden

抄録

<jats:title>Abstract</jats:title><jats:p>Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.</jats:p>

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