Interbase FRET in RNA: from A to Z
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- Anders F Füchtbauer
- Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
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- Moa S Wranne
- Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
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- Mattias Bood
- Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-412 96, Sweden
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- Erik Weis
- Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-412 96, Sweden
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- Pauline Pfeiffer
- Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
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- Jesper R Nilsson
- Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
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- Anders Dahlén
- Medicinal Chemistry, Research and Early Development Cardiovascular, Renal and Metabolism, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
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- Morten Grøtli
- Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-412 96, Sweden
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- L Marcus Wilhelmsson
- Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
Abstract
<jats:title>Abstract</jats:title><jats:p>Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.</jats:p>
Journal
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- Nucleic Acids Research
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Nucleic Acids Research 47 (19), 9990-9997, 2019-09-23
Oxford University Press (OUP)
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Keywords
Details 詳細情報について
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- CRID
- 1361699994970703104
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- ISSN
- 13624962
- 03051048
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- Data Source
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- Crossref