Glycan:glycan interactions: High affinity biomolecular interactions that can mediate binding of pathogenic bacteria to host cells
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- Christopher J. Day
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Elizabeth N. Tran
- School of Biological Sciences, Department of Molecular and Cellular Biology, University of Adelaide, Adelaide, SA 5005, Australia;
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- Evgeny A. Semchenko
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Greg Tram
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Lauren E. Hartley-Tassell
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Preston S. K. Ng
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Rebecca M. King
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Rachel Ulanovsky
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Sarah McAtamney
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Michael A. Apicella
- Department of Microbiology, University of Iowa, Iowa City, IA 52242
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- Joe Tiralongo
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Renato Morona
- School of Biological Sciences, Department of Molecular and Cellular Biology, University of Adelaide, Adelaide, SA 5005, Australia;
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- Victoria Korolik
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
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- Michael P. Jennings
- Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia;
抄録
<jats:title>Significance</jats:title> <jats:p>Pathogens use cell surface carbohydrates as a means of attachment to host tissues. In several pathogenic bacteria, truncation of surface carbohydrates, lipooligosaccharide, or lipopolysaccharide have been reported to significantly reduce bacterial adherence to host cells. Here, we show that the lipooligosaccharide/lipopolysaccharide of four distinct bacterial pathogens bind directly to a range of host glycans. Surface plasmon resonance data confirmed binding among 66 different host–glycan:bacterial–glycan pairs. We also demonstrated that bacterial adherence can be competitively inhibited by either host cell or bacterial glycans. Our discovery of high-affinity glycan:glycan interactions in infectious disease may provide new approaches for therapy and prevention. The discovery of the existence of extensive, high-affinity interactions between glycans will alter the perception of the importance of these macromolecular interactions in all biological systems.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 112 (52), E7266-, 2015-12-16
Proceedings of the National Academy of Sciences