Optical methodology for detecting histologically unapparent nanoscale consequences of genetic alterations in biological cells
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- Hariharan Subramanian
- Departments of aBiomedical Engineering and
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- Prabhakar Pradhan
- Departments of aBiomedical Engineering and
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- Yang Liu
- Departments of aBiomedical Engineering and
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- Ilker R. Capoglu
- Departments of aBiomedical Engineering and
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- Xu Li
- Departments of aBiomedical Engineering and
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- Jeremy D. Rogers
- Departments of aBiomedical Engineering and
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- Alexander Heifetz
- Departments of aBiomedical Engineering and
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- Dhananjay Kunte
- Department of Internal Medicine, Evanston Northwestern Healthcare, Evanston, IL 60208
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- Hemant K. Roy
- Department of Internal Medicine, Evanston Northwestern Healthcare, Evanston, IL 60208
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- Allen Taflove
- Electrical Engineering and Computer Science, Northwestern University, Evanston, IL 60208; and
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- Vadim Backman
- Departments of aBiomedical Engineering and
書誌事項
- 公開日
- 2008-12-23
- DOI
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- 10.1073/pnas.0804723105
- 公開者
- National Academy of Sciences
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説明
<jats:p>Recently, there has been a major thrust to understand biological processes at the nanoscale. Optical microscopy has been exceedingly useful in imaging cell microarchitecture. Characterization of cell organization at the nanoscale, however, has been stymied by the lack of practical means of cell analysis at these small scales. To address this need, we developed a microscopic spectroscopy technique, single-cell partial-wave spectroscopy (PWS), which provides insights into the statistical properties of the nanoscale architecture of biological cells beyond what conventional microscopy reveals. Coupled with the mesoscopic light transport theory, PWS quantifies the disorder strength of intracellular architecture. As an illustration of the potential of the technique, in the experiments with cell lines and an animal model of colon carcinogenesis we show that increase in the degree of disorder in cell nanoarchitecture parallels genetic events in the early stages of carcinogenesis in otherwise microscopically/histologically normal-appearing cells. These data indicate that this advance in single-cell optics represented by PWS may have significant biomedical applications.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 105 (51), 20118-20123, 2008-12-23
National Academy of Sciences
