A nuclear protein NsiA from <i>Epichloë festucae</i> interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion
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- Aiko Tanaka
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
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- Shota Kamiya
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
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- Yoshino Ozaki
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
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- Shinichi Kameoka
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
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- Yuka Kayano
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
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- Sanjay Saikia
- School of Fundamental Sciences Massey University Palmerston North New Zealand
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- Fumitake Akano
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
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- Aiko Uemura
- Iwate Biotechnology Research Center Kitakami Japan
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- Hiroki Takagi
- Iwate Biotechnology Research Center Kitakami Japan
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- Ryohei Terauchi
- Iwate Biotechnology Research Center Kitakami Japan
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- Jun‐ichi Maruyama
- Department of Biotechnology The University of Tokyo Tokyo Japan
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- Hamzeh Haj Hammadeh
- Institut für Genetik Technische Universität Braunschweig Braunschweig Germany
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- André Fleissner
- Institut für Genetik Technische Universität Braunschweig Braunschweig Germany
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- Barry Scott
- School of Fundamental Sciences Massey University Palmerston North New Zealand
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- Daigo Takemoto
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan
書誌事項
- 公開日
- 2020-07-28
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/mmi.14568
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>The endophytic fungus <jats:italic>Epichloë festucae</jats:italic> systemically colonizes the intercellular spaces of cool‐season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes identified mutant FR405 that had an antagonistic interaction with the host plant. Perennial ryegrass infected with the FR405 mutant were stunted and underwent premature senescence and death. The disrupted gene in FR405 encodes a nuclear‐localized protein, designated as NsiA for nuclear protein for symbiotic infection. Like previously isolated symbiotic mutants the <jats:italic>nsiA</jats:italic> mutant is defective in hyphal cell fusion. NsiA interacts with Ste12, a C2H2 zinc‐finger transcription factor, and a MAP kinase MpkB. Both are known as essential components for cell fusion in other fungal species. In <jats:italic>E. festucae</jats:italic>, MpkB, but not Ste12, is essential for cell fusion. Expression of several genes required for cell fusion and symbiosis, including <jats:italic>proA/adv‐1</jats:italic>, <jats:italic>pro41/ham‐6</jats:italic>, <jats:italic>ham7, ham8,</jats:italic> and <jats:italic>ham9</jats:italic> were downregulated in the <jats:italic>nsiA</jats:italic> mutant. However, the NsiA ortholog in <jats:italic>Neurospora crassa</jats:italic> was not essential for hyphal cell fusion. These results demonstrate that the roles of NsiA and Ste12 orthologs in hyphal cell fusion are distinctive between fungal species.</jats:p>
収録刊行物
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- Molecular Microbiology
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Molecular Microbiology 114 (4), 626-640, 2020-07-28
Wiley
