Real-Time DNA Sequencing from Single Polymerase Molecules

DOI Web Site 被引用文献93件
  • John Eid
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Adrian Fehr
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Jeremy Gray
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Khai Luong
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • John Lyle
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Geoff Otto
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Paul Peluso
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • David Rank
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Primo Baybayan
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Brad Bettman
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Arkadiusz Bibillo
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Keith Bjornson
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Bidhan Chaudhuri
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Frederick Christians
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Ronald Cicero
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Sonya Clark
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Ravindra Dalal
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Alex deWinter
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • John Dixon
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Mathieu Foquet
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Alfred Gaertner
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Paul Hardenbol
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Cheryl Heiner
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Kevin Hester
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • David Holden
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Gregory Kearns
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Xiangxu Kong
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Ronald Kuse
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Yves Lacroix
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Steven Lin
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Paul Lundquist
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Congcong Ma
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Patrick Marks
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Mark Maxham
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Devon Murphy
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Insil Park
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Thang Pham
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Michael Phillips
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Joy Roy
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Robert Sebra
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Gene Shen
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Jon Sorenson
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Austin Tomaney
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Kevin Travers
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Mark Trulson
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • John Vieceli
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Jeffrey Wegener
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Dawn Wu
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Alicia Yang
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Denis Zaccarin
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Peter Zhao
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Frank Zhong
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Jonas Korlach
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.
  • Stephen Turner
    Pacific Biosciences, 1505 Adams Drive, Menlo Park, CA 94025, USA.

書誌事項

公開日
2009-01-02
DOI
  • 10.1126/science.1162986
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p>We present single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs). We detected the temporal order of their enzymatic incorporation into a growing DNA strand with zero-mode waveguide nanostructure arrays, which provide optical observation volume confinement and enable parallel, simultaneous detection of thousands of single-molecule sequencing reactions. Conjugation of fluorophores to the terminal phosphate moiety of the dNTPs allows continuous observation of DNA synthesis over thousands of bases without steric hindrance. The data report directly on polymerase dynamics, revealing distinct polymerization states and pause sites corresponding to DNA secondary structure. Sequence data were aligned with the known reference sequence to assay biophysical parameters of polymerization for each template position. Consensus sequences were generated from the single-molecule reads at 15-fold coverage, showing a median accuracy of 99.3%, with no systematic error beyond fluorophore-dependent error rates.</jats:p>

収録刊行物

  • Science

    Science 323 (5910), 133-138, 2009-01-02

    American Association for the Advancement of Science (AAAS)

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