A Spontaneous 3D Bone‐On‐a‐Chip for Bone Metastasis Study of Breast Cancer Cells
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- Sijie Hao
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
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- Laura Ha
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
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- Gong Cheng
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
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- Yuan Wan
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
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- Yiqiu Xia
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
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- Donna M. Sosnoski
- Department of Biochemistry and Molecular Biology The Pennsylvania State University University Park PA 16802 USA
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- Andrea M. Mastro
- Department of Biochemistry and Molecular Biology The Pennsylvania State University University Park PA 16802 USA
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- Si‐Yang Zheng
- Department of Biomedical Engineering Micro & Nano Integrated Biosystem (MINIBio) Laboratory The Pennsylvania State University University Park PA 16802 USA
書誌事項
- 公開日
- 2018-02-05
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/smll.201702787
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>Bone metastasis occurs at ≈70% frequency in metastatic breast cancer. The mechanisms used by tumors to hijack the skeleton, promote bone metastases, and confer therapeutic resistance are poorly understood. This has led to the development of various bone models to investigate the interactions between cancer cells and host bone marrow cells and related physiological changes. However, it is challenging to perform bone studies due to the difficulty in periodic sampling. Herein, a bone‐on‐a‐chip (BC) is reported for spontaneous growth of a 3D, mineralized, collagenous bone tissue. Mature osteoblastic tissue of up to 85 µm thickness containing heavily mineralized collagen fibers naturally formed in 720 h without the aid of differentiation agents. Moreover, co‐culture of metastatic breast cancer cells is examined with osteoblastic tissues. The new bone‐on‐a‐chip design not only increases experimental throughput by miniaturization, but also maximizes the chances of cancer cell interaction with bone matrix of a concentrated surface area and facilitates easy, frequent observation. As a result, unique hallmarks of breast cancer bone colonization, previously confirmed only in vivo, are observed. The spontaneous 3D BC keeps the promise as a physiologically relevant model for the in vitro study of breast cancer bone metastasis.</jats:p>
収録刊行物
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- Small
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Small 14 (12), e1702787-, 2018-02-05
Wiley
