Comparative Analysis of DNA Repair in Stem and Nonstem Glioma Cell Cultures
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- Monica Ropolo
- 1Molecular Mutagenesis and DNA Repair,
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- Antonio Daga
- 2Gene Transfer,
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- Fabrizio Griffero
- 2Gene Transfer,
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- Mara Foresta
- 1Molecular Mutagenesis and DNA Repair,
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- Gianluigi Casartelli
- 1Molecular Mutagenesis and DNA Repair,
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- Annalisa Zunino
- 3Cytogenetics, and
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- Alessandro Poggi
- 4Immunology Laboratories, Istituto Nazionale Ricerca Cancro;
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- Enrico Cappelli
- 5Department of Pediatric Hemato-Oncology, Istituto Giannina Gaslini;
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- Gianluigi Zona
- 6Section of Neurosurgery, Department of Neuroscience, Ophthalmology and Genetics, and
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- Renato Spaziante
- 6Section of Neurosurgery, Department of Neuroscience, Ophthalmology and Genetics, and
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- Giorgio Corte
- 2Gene Transfer,
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- Guido Frosina
- 1Molecular Mutagenesis and DNA Repair,
Description
<jats:title>Abstract</jats:title> <jats:p>It has been reported that cancer stem cells may contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. We have examined DNA repair in five stem and nonstem glioma cell lines. The population doubling time was significantly increased in stem compared with nonstem cells, and enhanced activation of Chk1 and Chk2 kinases was observed in untreated CD133+ compared with CD133− cells. Neither DNA base excision or single-strand break repair nor resolution of pH2AX nuclear foci were increased in CD133+ compared with CD133− cells. We conclude that glioma stem cells display elongated cell cycle and enhanced basal activation of checkpoint proteins that might contribute to their radioresistance, whereas enhanced DNA repair is not a common feature of these cells. (Mol Cancer Res 2009;7(3):383–92)</jats:p>
Journal
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- Molecular Cancer Research
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Molecular Cancer Research 7 (3), 383-392, 2009-03-17
American Association for Cancer Research (AACR)
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Details 詳細情報について
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- CRID
- 1361699995977705600
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- ISSN
- 15573125
- 15417786
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- Data Source
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- Crossref