Comparative Study of Size, Total Protein, Fibrinogen and 5-HT Content of Human and Canine Platelet Density Subpopulations

DOI PDF 1 Citations
  • Diego Mezzano
    The Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile
  • Eduardo Aranda
    The Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile
  • Arnaldo Foradori
    The Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile

Search this article

Description

<jats:title>Summary</jats:title><jats:p>The size, total protein, fibrinogen and 5-HT content were evaluated in density subpopulations of human and canine platelets fractionated in linear arabinogalactan gradients. The methodology was assessed to ascertain that platelet separation was by density and to discard artifactual changes and platelet release during the procedure. EDTA or PGEi increased the size of human PRP-platelets, but not of dog platelets. In humans, high density (HD) platelets were 1.26 times larger and contained 1.88 times more fibrinogen, 2.23 times more 5-HT and 1.37 times more protein than low density (LD) platelets; in dogs, these density cohorts did not differ in protein content, but LD platelets were 1.29 times larger and had 1.33 times more fibrinogen and 5-HT than HD platelets. These findings suggest that cell density is mostly dependent on the protein content per unit volume of platelets (and not on dense bodies). The differences in fibrinogen and 5-HT content between HD and LD cohorts in humans and dogs may be related to platelet age. The difference in volume between HD and LD platelets in dogs is of uncertain interpretation.</jats:p>

Journal

Citations (1)*help

See more

Details 詳細情報について

Report a problem

Back to top