Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation

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  • Jemma N. Roberts
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Jugal Kishore Sahoo
    Department of Pure & Applied Chemistry, WestCHEM, Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, Scotland, U.K.
  • Laura E. McNamara
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Karl V. Burgess
    Glasgow Polyomics Facility, Translational Cancer Research Centre, University of Glasgow Garscube Campus, Switchback Road, Glasgow G61 1QH, Scotland, U.K.
  • Jingli Yang
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Enateri V. Alakpa
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Hilary J. Anderson
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Jake Hay
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Lesley-Anne Turner
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Stephen J. Yarwood
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.
  • Mischa Zelzer
    School of Pharmacy, University of Nottingham, Boots Science Building, University Park, Nottingham NG7 2RD, U.K.
  • Richard O. C. Oreffo
    Bone & Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, U.K.
  • Rein V. Ulijn
    Department of Pure & Applied Chemistry, WestCHEM, Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, Scotland, U.K.
  • Matthew J. Dalby
    Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.

書誌事項

公開日
2016-06-27
権利情報
  • http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html
DOI
  • 10.1021/acsnano.6b01765
公開者
American Chemical Society (ACS)

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説明

Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study, we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation, hence better informing biological mechanisms of stem cell growth. Tools that are able to investigate the stem cell phenotype are important. While large phenotypical differences, such as the difference between an adipocyte and an osteoblast, are now better understood, the far more subtle differences between fibroblasts and MSCs are much harder to dissect. The development of technologies able to dynamically navigate small differences in adhesion are critical in the race to provide regenerative strategies using stem cells.

収録刊行物

  • ACS Nano

    ACS Nano 10 (7), 6667-6679, 2016-06-27

    American Chemical Society (ACS)

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