In Vitro Cultivation of Microsporidia of Clinical Importance

DOI Web Site 被引用文献2件
  • Govinda S. Visvesvara
    Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724

書誌事項

公開日
2002-07
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/cmr.15.3.401-413.2002
公開者
American Society for Microbiology

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<jats:sec> <jats:title>SUMMARY</jats:title> <jats:p> Although attempts to develop methods for the in vitro cultivation of microsporidia began as early as 1937, the interest in the culture of these organisms was confined mostly to microsporidia that infect insects. The successful cultivation in 1969 of <jats:italic>Encephalitozoon cuniculi</jats:italic> , a microsporidium of mammalian origin, and the subsequent identification of these organisms as agents of human disease heightened interest in the cultivation of microsporidia. I describe the methodology as well as the cell lines, the culture media, and culture conditions used in the in vitro culture of microsporidia such as <jats:italic>Brachiola (Nosema) algerae, Encephalitozoon cuniculi, E. hellem, E. intestinalis, Enterocytozoon bieneusi, Trachipleistophora hominis,</jats:italic> and <jats:italic>Vittaforma corneae</jats:italic> that cause human disease. </jats:p> </jats:sec>

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