Majority of CD4 <sup>+</sup> T cells from peripheral blood of HIV-1–infected individuals contain only one HIV DNA molecule
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- Lina Josefsson
- Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;
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- Martin S. King
- Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60069;
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- Barbro Makitalo
- Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;
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- Johan Brännström
- Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;
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- Wei Shao
- Advanced Biomedical Computing Center, SAIC Frederick, Inc., Frederick, MD 21702; and
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- Frank Maldarelli
- HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;
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- Mary F. Kearney
- HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;
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- Wei-Shau Hu
- HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;
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- Jianbo Chen
- HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;
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- Hans Gaines
- Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;
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- John W. Mellors
- Division of Infectious Diseases, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261
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- Jan Albert
- Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, 171 82, Solna, Sweden;
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- John M. Coffin
- HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702;
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- Sarah E. Palmer
- Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82, Solna, Sweden;
書誌事項
- 公開日
- 2011-06-20
- DOI
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- 10.1073/pnas.1107729108
- 公開者
- National Academy of Sciences
この論文をさがす
説明
<jats:p> Neither the number of HIV-1 proviruses within individual infected cells in HIV-1–infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4 <jats:sup>+</jats:sup> T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4 <jats:sup>+</jats:sup> T cells and plasma implies ongoing exchange between these compartments both early and late after infection. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 108 (27), 11199-11204, 2011-06-20
National Academy of Sciences
