Sperm rescue in wild African elephants

DOI PDF 被引用文献1件
  • Joseph Saragusty
    ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, D-10315 Berlin, Germany.
  • María Teresa Prieto
    ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, D-10315 Berlin, Germany.
  • Alexandre Courtiol
    ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, D-10315 Berlin, Germany.
  • Romain Potier
    BZooParc de Beauval, 41110 Saint Aignan s/ Cher, France.
  • Frank Göritz
    ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, D-10315 Berlin, Germany.
  • Thomas B. Hildebrandt
    ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, D-10315 Berlin, Germany.
  • Robert Hermes
    ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, D-10315 Berlin, Germany.

書誌事項

公開日
2015-03-13
権利情報
  • https://doi.org/10.1071/journalslicense
DOI
  • 10.1071/rd14378
公開者
CSIRO Publishing

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説明

<jats:p>This study investigated ways of improving the usefulness of ~1700 mL of poor-quality frozen semen collected from wild African elephant (Loxodonta africana) bulls. Ten semen samples from six bulls, frozen with 5% glycerol in Berliner cryomedium, with or without prior removal of the seminal plasma by centrifugation, were tested. All samples were subjected to the following density-gradient centrifugation treatments: no centrifugation (control), sham centrifugation, Percoll, OptiPrep, Isolate and PureSperm. Sample evaluation included motility, concentration, viability, acrosome integrity and normal morphology after thawing and after gradient centrifugation. Motility was also evaluated 3 h after thawing. While all treatments were similar to the Control in acrosome integrity and normal morphology, significant differences were noted in concentration, viability and motility. Samples treated by Percoll showed the best motility, which was maintained unchanged over 3 h of incubation (37°C). Correlations between manual and automated evaluations of concentration were high (cytometer; rho = 0.92), but were lower for viability (cytometer; rho = 0.57) and motility (computer-aided sperm analysis; rho = 0.66). By performing density centrifugation, the quality of these sperm samples may be improved to a level suitable for artificial insemination in elephants. Although a sizeable proportion of cells are lost in the process, combining samples may still allow for multiple inseminations.</jats:p>

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