LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 Expression in Human Lymphatic Endothelium

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  • Yoshihiko Sawa
    Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
  • Takeshi Ueki
    Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan
  • Minoru Hata
    Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan
  • Kana Iwasawa
    Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan
  • Eichi Tsuruga
    Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
  • Hiroshi Kojima
    Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan
  • Hiroyuki Ishikawa
    Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan
  • Shigemitsu Yoshida
    Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

書誌事項

公開日
2007-10-15
権利情報
  • https://journals.sagepub.com/page/policies/text-and-data-mining-license
DOI
  • 10.1369/jhc.7a7299.2007
公開者
SAGE Publications

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<jats:p>We have previously reported the TLR4 expression inhuman intestinal lymphatic vessels. In the study here, microarray analysis showed the expression of the TLR4, MD-2, CD14, MyD88, TIRAP, TRAM, IRAK1, and TRAF6 genes in cultured human neonatal dermal lymphatic microvascular endothelial cells (LEC). The microarray analysis also showed that LEC expressed genes of IL-6, IL-8, VCAM-1, and ICAM-1, and the real-time quantitative PCR analysis showed that mRNA production was increased by lipopolysaccharide (LPS). The LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 production in LEC was suppressed by the introduction of TLR4-specific small interfering RNA, and also by anti-TLR4, nobiletin, and CAPE pretreatment. These findings suggest that LEC has TLR4-mediated LPS recognition mechanisms that involve at least activation of NF-κB, resulting in increased expression of IL-6, IL-8, VCAM-1, and ICAM-1. Both the LPS effect on the gene expression and also the suppression by nobiletin and CAPE pre-treatment on the protein production were larger in IL-6 and in VCAM-1 than in IL-8 and in ICAM-1 in LEC. The signal transduction of NF-κB and AP-1-dependent pathway may be more critical for the expression of IL-6 and VCAM-1 than that of IL-8 and ICAM-1 in LEC.</jats:p>

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