FITC and Ru(phen)3 2+ co-doped silica particles as visualized ratiometric pH indicator
書誌事項
- 公開日
- 2011-10-25
- DOI
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- 10.1186/1556-276x-6-561
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title> <jats:p>The performance of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)<jats:sub>3</jats:sub> <jats:sup>2+</jats:sup>) co-doped silica particles as pH indicator was evaluated. The emission intensity ratios of the pH sensitive dye (FITC) and the reference dye (Ru(phen)<jats:sub>3</jats:sub> <jats:sup>2+</jats:sup>) in the particles were dependent on pH of the environment. The changes in emission intensity ratios of the two dyes under different pH could be measured under single excitation wavelength and readily visualized by naked eye under a 365-nm UV lamp. In particular, such FITC and Ru(phen)<jats:sub>3</jats:sub> <jats:sup>2+</jats:sup> co-doped silica particles were identified to show high sensitivity to pH around the pKa of FITC (6.4), making them be potential useful as visualized pH indicator for detection of intracellular pH micro-circumstance.</jats:p>
収録刊行物
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- Nanoscale Research Letters
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Nanoscale Research Letters 6 (1), 561-, 2011-10-25
Springer Science and Business Media LLC