Toward a Consensus on Applying Quantitative Liquid Chromatography‐Tandem Mass Spectrometry Proteomics in Translational Pharmacology Research: A White Paper
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- Bhagwat Prasad
- Department of Pharmaceutics University of Washington Seattle Washington USA
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- Brahim Achour
- Centre for Applied Pharmacokinetic Research University of Manchester Manchester UK
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- Per Artursson
- Department of Pharmacy Uppsala University Uppsala Sweden
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- Cornelis E.C.A. Hop
- Genentech South San Francisco California USA
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- Yurong Lai
- Gilead Sciences Foster City California USA
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- Philip C. Smith
- Eshelman School of Pharmacy University of North Carolina at Chapel Hill Chapel Hill North Carolina USA
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- Jill Barber
- Centre for Applied Pharmacokinetic Research University of Manchester Manchester UK
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- Jacek R. Wisniewski
- Biochemical Proteomics Group Max Planck Institute of Biochemistry Martinsried Germany
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- Daniel Spellman
- Pharmacokinetics Pharmacodynamics & Drug Metabolism Merck & Co., Inc. West Point Pennsylvania USA
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- Yasuo Uchida
- Graduate School of Pharmaceutical Sciences Tohoku University Sendai Japan
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- Michael A. Zientek
- Takeda California San Diego California USA
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- Jashvant D. Unadkat
- Department of Pharmaceutics University of Washington Seattle Washington USA
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- Amin Rostami‐Hodjegan
- Centre for Applied Pharmacokinetic Research University of Manchester Manchester UK
説明
<jats:p>Quantitative translation of information on drug absorption, disposition, receptor engagement, and drug–drug interactions from bench to bedside requires models informed by physiological parameters that link <jats:italic>in vitro</jats:italic> studies to <jats:italic>in vivo</jats:italic> outcomes. To predict <jats:italic>in vivo</jats:italic> outcomes, biochemical data from experimental systems are routinely scaled using protein quantity in these systems and relevant tissues. Although several laboratories have generated useful quantitative proteomic data using state‐of‐the‐art mass spectrometry, no harmonized guidelines exit for sample analysis and data integration to <jats:italic>in vivo</jats:italic> translation practices. To address this gap, a workshop was held on September 27 and 28, 2018, in Cambridge, <jats:styled-content style="fixed-case">MA</jats:styled-content>, with 100 experts attending from academia, the pharmaceutical industry, and regulators. Various aspects of quantitative proteomics and its applications in translational pharmacology were debated. A summary of discussions and best practices identified by this expert panel are presented in this “White Paper” alongside unresolved issues that were outlined for future debates.</jats:p>
収録刊行物
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- Clinical Pharmacology & Therapeutics
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Clinical Pharmacology & Therapeutics 106 (3), 525-543, 2019-07-26
Wiley
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詳細情報 詳細情報について
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- CRID
- 1361975839489214336
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- DOI
- 10.1002/cpt.1537
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- ISSN
- 15326535
- 00099236
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- データソース種別
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- Crossref
- KAKEN